Metabolic-fluid dynamics model construction and scale-down design for an industrial penicillin chrysogenum fermentation with combined dissolved oxygen and glucose concentration dynamics

This study focuses on the metabolic impacts of simultaneous glucose and oxygen concentration gradients penicillin production in an industrial-scale fermentor, using computational fluid dynamics-cellular reaction dynamics approach. Inclusion oxygen-coupling considerably uptake resulting productivity. is characterised by six regimes; lifeline data reconstructed from experimental results, recorded cellular perspective, indicates rapid dissolved microorganisms. The results are highly sensitive to variations oxygen-related model parameters, requiring accurate insight into multiphase hydrodynamics processes. Hypothetical scenarios with stronger glucose-oxygen limitations than tested experimentally were further explored. A precision scale-down (SD) simulator was designed based data, considerable operational dynamics, increasing system complexity implementation difficulty. These insights may inspire research alternative SD configurations better suited mimic large-scale fermentation